Tag
TEV cut si

Part:BBa_K128002:Design

Designed by: Sara Mouradian   Group: iGEM08_MIT   (2008-07-10)


TEV tobacco etch virus protease cleavage site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The TEV cut site is almost always used in conjunction with other peptides, either to release the protein of interest from a binding assay, or to cut two proteins apart post-translationally. Because of this, we used the modified Silver BioBrick prefix and suffix to make the construction of funcitonal fusion peptides possible.


Source

This is the cut sequence for TEV protease put forth by the Dougherty Lab (North Carolina State University). The general form is E-X-X-Y-X-Q-(G/S), but ENLYFQS was found to be most efficient


References